寡妇好丰满奶好大有声小说,免费观看性生交大片 ,亚洲精品无码久久久久秋霞,爱的色放在线

您好,歡迎進(jìn)入上海力敏實(shí)業(yè)有限公司網(wǎng)站!
一鍵分享網(wǎng)站到:
您現(xiàn)在的位置:首頁 >> 產(chǎn)品中心 >> 生化試劑、抗體、血清 >> 抗體/蛋白 >> SC-2002santacruz G蛋白PLUS-瓊脂糖Protein G PLUS-Agarose

santacruz G蛋白PLUS-瓊脂糖Protein G PLUS-Agarose

  • 更新時(shí)間:  2023-07-25
  • 產(chǎn)品型號(hào):  SC-2002
  • 簡(jiǎn)單描述
  • Protein G PLUS-Agarose is suitable for immunoprecipitation of mouse IgG1,
    IgG2a, IgG2b and IgG3, rat IgG1, IgG2a, santacruz G蛋白PLUS-瓊脂糖Protein G PLUS-AgaroseIgG2b and IgG2c, rabbit and goat polycl
詳細(xì)介紹

santacruz  G蛋白PLUS-瓊脂糖Protein G PLUS-Agarose

SANTA CRUZ BIOTECHNOLOGY, INC.
Protein G PLUS-Agarose
Immunoprecipitation Reagent: sc-2002
Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com
PRODUCT
Protein G PLUS is provided as an agarose conjugate for use in immunoprecipitation
only. The product is provided as 0.5 ml agarose in 2.0 ml PBS buffer
with 0.02% azide. Protein G PLUS-Agarose is pre-blocked with BSA to reduce
non-specific immunoglobulin binding. Sufficient product is provided for 100
immunoprecipitation reactions, to be used at 20 μl resuspended volume per
reaction.
SPECIFICITY
Protein G PLUS-Agarose is suitable for immunoprecipitation of mouse IgG1,
IgG2a, IgG2b and IgG3, rat IgG1, IgG2a, IgG2b and IgG2c, rabbit and goat polyclonal
Abs, and human IgG1, IgG2, IgG3 and IgG4.
PROCEDURE
? Incubate cultured cells (80-90% confluent monolayer in 100 mm cell culture
plate, or approximay 2-5 x 107 suspension cells in flask) in methioninefree
medium containing 5% dialyzed fetal calf serum for 1 hour at 37° C.
The same procedure can be used for cells labeled with other radioactive
amino acids (e.g., 14C or 3H) or with γ32P-orthophosphate. Cell labeling must
be carried out in medium lacking the relevant amino acid or in phosphatefree
medium.
? Remove medium and replace with 3 ml methionine-free medium containing
5% dialyzed fetal calf serum and 100 μCi/ml 35S-methionine. Incubate 1
hour at 37° C. For some proteins a longer labeling period (up to 18 hours)
is preferable.
? Carefully remove radioactive medium with Pasteur pipette and wash cell
monolayer with PBS.
? Add 3 ml ice cold RIPA buffer to cell monolayer and incubate at 4° C for
10 minutes. For suspension cells, add the RIPA buffer to washed cell pellet
in a 15 ml conical centrifuge tube.
? Disrupt cells by repeated aspiration through a 21 gauge needle and transfer
to a 15 ml conical centrifuge tube.
? Wash cell culture plate with additional 1.0 ml ice cold RIPA buffer and
combine with original extract.
? Pellet cellular debris by centrifugation at 10,000 xg for 10 minutes at 4° C.
Transfer supernatant to a fresh 15 ml conical centrifuge tube on ice.
Preclear lysate (optional step) by adding 1.0 μg of the appropriate control
IgG (normal mouse, rat, rabbit or goat IgG, corresponding to the host
species of the primary antibody), together with 20 μl of resuspended
volume of Protein G PLUS-Agarose. Incubate at 4° C for 30 minutes.
? Pellet beads by centrifugation at 2,500 rpm (approximay 1,000 xg) for
5 minutes at 4° C. Transfer supernatant (cell lysate) to a fresh 15 ml conical
centrifuge tube on ice.
? Transfer 1 ml of the above cell lysate, or approximay 100-500 μg total
cellular protein, to a 1.5 ml microcentrifuge tube. Add 1-10 μl (i.e., 0.2-2 μg)
primary antibody (optimal antibody concentration should be determined by
titration) and incubate for 1 hour at 4° C.
? Add 20 μl of resuspended volume of Protein G PLUS-Agarose. Cap tubes
and incubate at 4° C on a rocker platform or rotating device for 1 hour to
overnight.
? Collect immunoprecipitates by centrifugation at 2,500 rpm (approximay
1,000 xg) for 5 minutes at 4° C. Carefully aspirate and discard radioactive
supernatant.
? Wash pellet 4 times with 1.0 ml RIPA buffer (more stringent) or PBS (less
stringent), each time repeating centrifugation step above.
? After final wash, aspirate and discard supernatant and resuspend pellet in
40 μl of 1x electrophoresis sample buffer.
? Boil samples for 2–3 minutes and analyze 20 μl aliquots by SDS-PAGE and
autoradiography. Unused samples may be stored at -20° C.
? Optional: After boiling, samples may be centrifuged to pellet the agarose
beads followed by SDS-PAGE analysis of the supernatant.
SELECT PRODUCT CITATIONS
1. Medema, R., et al. 1998. p21waf1 can block cells at two points in the cell
cycle, but does not interfere with processive DNA-replication or stressactivated
kinases. Oncogene 16: 431-441.
2. Marzo, I., et al. 1998. Bax and adenine nucleotide translocator cooperate
in the mitochondrial control of apoptosis. Science 281: 2027-2031.
3. Song, Y., et al. 2011. Ligand-dependent corepressor acts as a novel corepressor
of thyroid hormone receptor and represses hepatic lipogenesis in
mice. J. Hepatol. 56: 248-254.
4. Beard, R.S., et al. 2012. Metabotropic glutamate receptor 5 mediates
phosphorylation of vascular endothelial cadherin and nuclear localization
of β-catenin in response to homocysteine. Vascul. Pharmacol. 56: 159-167.
STORAGE
Store at 4° C, do not freeze; stable for one year from the date of shipment.
RESEARCH USE
For research use only, not for use in diagnostic procedures.
Immunoprecipitation agarose conjugates are pre-blocked with BSA to reduce non-specific immunoglobulin
binding and are provided at a concentration (0.5 ml agarose/2.0 ml) suitable for use at 20 μl per
immunoprecipitation reaction. Number of reactions: 100.

 

上海力敏實(shí)業(yè)有限公司代理santacruz產(chǎn)品,咨詢santacruz  G蛋白PLUS-瓊脂糖Protein G PLUS-Agarose 詳細(xì)產(chǎn)品信息


留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
日韩av无码| 久久精品国产久精国产| 69丰满少妇av无码区| 无码国产一区二区三区四区公司| 国产aⅴ激情无码久久久无码| 隔壁小寡妇让我爽了一夜| 日韩人妻无码一区二区三区免费| 扒开少年白嫩的屁股动态图| 5d肉蒲团之性战奶水又爽又黄| 国产精品久久久久久久久免费| 无码熟妇av人妻又粗又大| 国产精品视频一区| 麻豆av天堂一二三区视频| 亚洲6080yy久久无码国产 | 在床边趴着屁股撅起来怎么弄 | 亚洲av无码成人精品区在线观看 | 天美传媒剧国产剧情MV公司| 美国式禁忌| 学生16女人毛片免费视频| 在仓库玩50岁保洁女| 国产粉嫩小泬在线观看泬| 免费看国产曰批40分钟| 欧美亚洲一区二区三区| 无需付费看美女直播的软件| 青娱乐极品盛宴| 国产偷窥盗拍丰满老熟女| 我半夜摸妺妺的奶摸到高潮| 好猛好深好爽喷水无码视频| 久久久久亚洲av无码专区网站 | 粗大的内捧猛烈进出| 体育生gay白袜调教video| 白领人妻系列第26部分阅读| 色视频www在线播放国产人成| 白洁性荡生活第90章| 高雅人妻被迫沦为玩物| 50岁退休熟女露脸高潮| 玩弄六个女邻居| 亚洲成色www久久网站| 国产又粗又猛又爽又黄| 女生裸妆bb| chinesevideo国产熟妇|